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protein concentration measurement  (Thermo Fisher)


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    Structured Review

    Thermo Fisher protein concentration measurement
    Protein Concentration Measurement, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/protein+concentration+measurement/BCA+Protein+Assay+Kit/pmc12890482-245-15-18
    Average 99 stars, based on 1 article reviews
    protein concentration measurement - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    other:

    Article Title: Revealing the Mechanism of NLRP3 Inflammatory Pathway Activation through K + Efflux Induced by PLO via Signal Point Mutations.
    Article Snippet: The protein concentration was measured by Nano Drop (ND-ONE-W, Thermo Fisher, Waltham, MA, USA), and the purity was then detected by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE).

    Article Title: Natural Antimicrobials Block the Host NF-κB Pathway and Reduce Enterocytozoon hepatopenaei Infection Both In Vitro and In Vivo.
    Article Snippet: Briefly, prior to separation in 10% SDS polyacrylamide gels, the protein concentration was measured (Thermo Fisher, Horsham, UK) and denatured in 1× Laemmli buffer (Sigma-Aldrich, Gillingham, UK).

    Article Title: Molecular mechanism of human α 1A -adrenoceptor inhibition by Mamba snake toxin AdTx1
    Article Snippet: The protein concentration was measured using a Nanodrop (Thermo).

    Article Title: Structural and Functional Characterization of the Most Frequent Pathogenic PRKN Substitution p.R275W
    Article Snippet: Samples were spun (21,000× g , 4 °C, 15 min), the supernatant saved, and protein concentration was measured (Thermo Fisher Scientific, 23225).

    Protein Concentration:

    Article Title: Gc inhibition preserves insulin sensitivity and reduces body weight without loss of muscle mass.
    Article Snippet: The supernatant was sonicated for a total of 5 minutes (10 seconds on and 10 seconds off for 10 minutes) on setting 9 of a model 550 Sonic Dismembrator (Fisher Scientific). .. The supernatant was spun again and transferred to a new tube avoiding precipitant, followed by protein concentration measurement (Pierce BCA protein assay kit). .. The following primary antibodies were used: anti-Gc (HPA019855, Atlas Antibodies, 1:1000 dilution); anti-total Akt (Cell Signaling, no. 9272, 1:1000 dilution); anti-phosphorylated Akt at serine 473 (Cell Signaling, no. 4060, 1:500 dilution); and anti-β actin (Abcam, ab8227, 1:1000 dilution).

    Article Title: Pcbp1 constrains Oct4 expression in the context of pluripotency
    Article Snippet: .. Aliquot (1/10) of this mixture was used for protein concentration measurement by PierceTM BCA Protein Assay Kit (ThermoFisher Scientific). ..

    Article Title: Nerve-independent formation of membrane infoldings at topologically complex postsynaptic apparatus by caveolin-3.
    Article Snippet: .. Nieuwkoop and Faber stage 19 to 22 Xenopus embryos were homogenized in radioimmunoprecipitation assay buffer in the presence of protease inhibitor cocktail and EDTA, followed by incubation on ice for 5 min. After high-speed centrifugation (15,000g), the supernatant was collected for protein concentration measurement using a BCA protein assay kit (Thermo Fisher Scientific, 23227). .. Protein lysates (30 μg) were used for protein separation by SDS–polyacrylamide gel electrophoresis (SDS-PAGE) and then blotted onto polyvinylidene difluoride membranes (Bio-Rad, 1620174).

    Article Title: Gc inhibition preserves insulin sensitivity and reduces body weight without loss of muscle mass
    Article Snippet: The supernatant was sonicated for a total of 5 minutes (10 seconds on and 10 seconds off for 10 minutes) on setting 9 of a model 550 Sonic Dismembrator (Fisher Scientific). .. The supernatant was spun again and transferred to a new tube avoiding precipitant, followed by protein concentration measurement (Pierce BCA protein assay kit). .. The following primary antibodies were used: anti-Gc (HPA019855, Atlas Antibodies, 1:1000 dilution); anti-total Akt (Cell Signaling, no. 9272, 1:1000 dilution); anti-phosphorylated Akt at serine 473 (Cell Signaling, no. 4060, 1:500 dilution); and anti-β actin (Abcam, ab8227, 1:1000 dilution).

    Bicinchoninic Acid Protein Assay:

    Article Title: Gc inhibition preserves insulin sensitivity and reduces body weight without loss of muscle mass.
    Article Snippet: The supernatant was sonicated for a total of 5 minutes (10 seconds on and 10 seconds off for 10 minutes) on setting 9 of a model 550 Sonic Dismembrator (Fisher Scientific). .. The supernatant was spun again and transferred to a new tube avoiding precipitant, followed by protein concentration measurement (Pierce BCA protein assay kit). .. The following primary antibodies were used: anti-Gc (HPA019855, Atlas Antibodies, 1:1000 dilution); anti-total Akt (Cell Signaling, no. 9272, 1:1000 dilution); anti-phosphorylated Akt at serine 473 (Cell Signaling, no. 4060, 1:500 dilution); and anti-β actin (Abcam, ab8227, 1:1000 dilution).

    Article Title: Pcbp1 constrains Oct4 expression in the context of pluripotency
    Article Snippet: .. Aliquot (1/10) of this mixture was used for protein concentration measurement by PierceTM BCA Protein Assay Kit (ThermoFisher Scientific). ..

    Article Title: Nerve-independent formation of membrane infoldings at topologically complex postsynaptic apparatus by caveolin-3.
    Article Snippet: .. Nieuwkoop and Faber stage 19 to 22 Xenopus embryos were homogenized in radioimmunoprecipitation assay buffer in the presence of protease inhibitor cocktail and EDTA, followed by incubation on ice for 5 min. After high-speed centrifugation (15,000g), the supernatant was collected for protein concentration measurement using a BCA protein assay kit (Thermo Fisher Scientific, 23227). .. Protein lysates (30 μg) were used for protein separation by SDS–polyacrylamide gel electrophoresis (SDS-PAGE) and then blotted onto polyvinylidene difluoride membranes (Bio-Rad, 1620174).

    Article Title: Gc inhibition preserves insulin sensitivity and reduces body weight without loss of muscle mass
    Article Snippet: The supernatant was sonicated for a total of 5 minutes (10 seconds on and 10 seconds off for 10 minutes) on setting 9 of a model 550 Sonic Dismembrator (Fisher Scientific). .. The supernatant was spun again and transferred to a new tube avoiding precipitant, followed by protein concentration measurement (Pierce BCA protein assay kit). .. The following primary antibodies were used: anti-Gc (HPA019855, Atlas Antibodies, 1:1000 dilution); anti-total Akt (Cell Signaling, no. 9272, 1:1000 dilution); anti-phosphorylated Akt at serine 473 (Cell Signaling, no. 4060, 1:500 dilution); and anti-β actin (Abcam, ab8227, 1:1000 dilution).

    Radio Immunoprecipitation:

    Article Title: Nerve-independent formation of membrane infoldings at topologically complex postsynaptic apparatus by caveolin-3.
    Article Snippet: .. Nieuwkoop and Faber stage 19 to 22 Xenopus embryos were homogenized in radioimmunoprecipitation assay buffer in the presence of protease inhibitor cocktail and EDTA, followed by incubation on ice for 5 min. After high-speed centrifugation (15,000g), the supernatant was collected for protein concentration measurement using a BCA protein assay kit (Thermo Fisher Scientific, 23227). .. Protein lysates (30 μg) were used for protein separation by SDS–polyacrylamide gel electrophoresis (SDS-PAGE) and then blotted onto polyvinylidene difluoride membranes (Bio-Rad, 1620174).

    Protease Inhibitor:

    Article Title: Nerve-independent formation of membrane infoldings at topologically complex postsynaptic apparatus by caveolin-3.
    Article Snippet: .. Nieuwkoop and Faber stage 19 to 22 Xenopus embryos were homogenized in radioimmunoprecipitation assay buffer in the presence of protease inhibitor cocktail and EDTA, followed by incubation on ice for 5 min. After high-speed centrifugation (15,000g), the supernatant was collected for protein concentration measurement using a BCA protein assay kit (Thermo Fisher Scientific, 23227). .. Protein lysates (30 μg) were used for protein separation by SDS–polyacrylamide gel electrophoresis (SDS-PAGE) and then blotted onto polyvinylidene difluoride membranes (Bio-Rad, 1620174).

    Incubation:

    Article Title: Nerve-independent formation of membrane infoldings at topologically complex postsynaptic apparatus by caveolin-3.
    Article Snippet: .. Nieuwkoop and Faber stage 19 to 22 Xenopus embryos were homogenized in radioimmunoprecipitation assay buffer in the presence of protease inhibitor cocktail and EDTA, followed by incubation on ice for 5 min. After high-speed centrifugation (15,000g), the supernatant was collected for protein concentration measurement using a BCA protein assay kit (Thermo Fisher Scientific, 23227). .. Protein lysates (30 μg) were used for protein separation by SDS–polyacrylamide gel electrophoresis (SDS-PAGE) and then blotted onto polyvinylidene difluoride membranes (Bio-Rad, 1620174).

    Centrifugation:

    Article Title: Nerve-independent formation of membrane infoldings at topologically complex postsynaptic apparatus by caveolin-3.
    Article Snippet: .. Nieuwkoop and Faber stage 19 to 22 Xenopus embryos were homogenized in radioimmunoprecipitation assay buffer in the presence of protease inhibitor cocktail and EDTA, followed by incubation on ice for 5 min. After high-speed centrifugation (15,000g), the supernatant was collected for protein concentration measurement using a BCA protein assay kit (Thermo Fisher Scientific, 23227). .. Protein lysates (30 μg) were used for protein separation by SDS–polyacrylamide gel electrophoresis (SDS-PAGE) and then blotted onto polyvinylidene difluoride membranes (Bio-Rad, 1620174).



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